Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GSK3 beta antibody [C1C3] diluted at 1:500.| Product Name | GSK3 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSK3 beta (glycogen synthase kinase 3 beta)Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GSK3 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
|
Not tested in other applications. : |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Pig, Sheep, BovineZebrafish (83%), Pig (90%), Sheep (90%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSK3B antibody GSK-3 beta antibody serine/threonine-protein kinase GSK3B antibody glycogen synthase kinase-3 beta antibody GSK3beta isoform antibody glycogen synthase kinase 3 beta antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GSK3 beta antibody [C1C3] diluted at 1:500.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes |
Not tested in other applications. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
