Application
GSTM5 antibody detects GSTM5 protein at cytoplasm in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. GSTM5 antibody diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse testis 12% SDS PAGE diluted at 1:10000Application
Sample (50 ug of whole cell lysate) A: rat testis 12% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GST Mu5 antibody at 1:200 dilution.| Product Name | GSTM5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSTM5 (glutathione S-transferase Mu 5)Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. [provided by RefSeq] |
| Immunogen | Full length human GSTM5 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSTM5-5 antibody GTM5 antibody GSTM5 antibody S-(hydroxyalkyl)glutathione lyase M5 antibody glutathione S-aryltransferase M5 antibody GST class-mu 5 antibody glutathione S-alkyltransferase M5 antibody glutathione S-aralkyltransferase M5 antibody gl |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
GSTM5 antibody detects GSTM5 protein at cytoplasm in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. GSTM5 antibody diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse testis 12% SDS PAGE diluted at 1:10000
Application
Sample (50 ug of whole cell lysate) A: rat testis 12% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GST Mu5 antibody at 1:200 dilution.| Positive Control | mouse testis , rat testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
