Application
GSTT1 antibody detects GSTT1 protein by western blot analysis. A. 50 µg mouse spleen lysate/extract 12% SDS-PAGE GSTT1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GSTT1 antibody detects GSTT1 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. GSTT1 antibody dilution: 1:500.Application
GSTT1 antibody detects GSTT1 protein by western blot analysis. A. 50 µg rat liver lysate/extract 12% SDS-PAGE GSTT1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 μg of GSTT1 antibody . Western blot analysis was performed using GSTT1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | GSTT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSTT1 (glutathione S-transferase theta 1)Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq] |
| Immunogen | Full length human GSTT1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (97%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSTT1 antibody glutathione transferase T1-1 antibody GST class-theta-1 antibody glutathione S-transferase theta-1 antibody glutathione S-transferase theta 1 antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
GSTT1 antibody detects GSTT1 protein by western blot analysis. A. 50 µg mouse spleen lysate/extract 12% SDS-PAGE GSTT1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GSTT1 antibody detects GSTT1 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. GSTT1 antibody dilution: 1:500.
Application
GSTT1 antibody detects GSTT1 protein by western blot analysis. A. 50 µg rat liver lysate/extract 12% SDS-PAGE GSTT1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 μg of GSTT1 antibody . Western blot analysis was performed using GSTT1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | HepG2 , mouse spleen , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
