Application
GTF2IRD1 antibody detects GTF2IRD1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GTF2IRD1 protein stained by GTF2IRD1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GTF2IRD1 antibody diluted at 1:500.| Product Name | GTF2IRD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GTF2IRD1 (GTF2I repeat domain containing 1)The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing of this gene generates at least 2 transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GTF2IRD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (85%), Rat (85%), Rhesus Monkey (99%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BEN antibody CREAM1 antibody GTF2I repeat domain containing 1 antibody GTF2I repeat domain-containing protein 1 antibody GTF2IRD1 antibody GTF2IRD1 antibody GTF3 antibody MUSTRD1 antibody RBAP2 antibody WBS antibody WBSCR11 an |
|---|---|
| Target | GTF2IRD1 |
| Molecular Weight(MW) | 106 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
GTF2IRD1 antibody detects GTF2IRD1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GTF2IRD1 protein stained by GTF2IRD1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GTF2IRD1 antibody diluted at 1:500.| Positive Control | HeLa , HeLa nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
