Application
Sample (10 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:5000Application
GTPBP4 antibody detects GTPBP4 protein at nucleolus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: GTPBP4 protein stained by GTPBP4 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
GTPBP4 antibody detects GTPBP4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE GTPBP4 antibody dilution: 1:5000| Product Name | GTPBP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GTPBP4 (GTP binding protein 4)GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GTPBP4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (88%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRFG antibody FLJ10686 antibody FLJ10690 antibody FLJ39774 antibody NGB antibody NOG1 antibody GTPBP4 antibody GTP-binding protein NGB antibody G protein-binding protein CRFG antibody nucleolar GTP-binding protein 1 antibody chronic renal failure |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) |
| Cellular Localization | Nucleus , nucleolus |
Application
Sample (10 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:5000
Application
GTPBP4 antibody detects GTPBP4 protein at nucleolus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: GTPBP4 protein stained by GTPBP4 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
GTPBP4 antibody detects GTPBP4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE GTPBP4 antibody dilution: 1:5000| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
