Application
Sample(30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:1000Application
HACL1 antibody detects HACL1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HACL1 antibody dilution: 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HACL1 antibody at 1:200 dilution.Application
HACL1 antibody detects HACL1 protein at cytosol on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded human normal liver. HACL1 antibody dilution: 1:500.| Product Name | HACL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HACL1 (2-hydroxyacyl-CoA lyase 1)Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HACL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chimpanzee, BovineRat (86%), Pig (85%), Chimpanzee (100%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 2-HPCL antibody FLJ55041 antibody HPCL antibody HPCL2 antibody PHYH2 antibody HACL1 antibody 2-hydroxyphytanol-CoA lyase antibody phytanoyl-CoA 2-hydroxylase 2 antibody 2-hydroxyacyl-CoA lyase 1 antibody 1600020H07Rik antibody 2-hydroxyphytanoyl- |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) (4) |
| Cellular Localization | Peroxisome |
Application
Sample(30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:1000
Application
HACL1 antibody detects HACL1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HACL1 antibody dilution: 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HACL1 antibody at 1:200 dilution.
Application
HACL1 antibody detects HACL1 protein at cytosol on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded human normal liver. HACL1 antibody dilution: 1:500.| Positive Control | 293T , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
