Application
Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 μg of HADH antibody . Western blot analysis was performed using HADH antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
HADH antibody detects HADH protein at mitochondria on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HADH antibody dilution: 1:500.Application
HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody dilution: 1:500.Application
HADH antibody detects HADH protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE HADH antibody dilution: 1:1000Application
HADH antibody detects HADH protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE HADH antibody dilution: 1:1000Application
HADH antibody detects HADH protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HADH protein stained by HADH antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
HADH antibody detects HADH protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE HADH antibody dilution: 1:1000| Product Name | HADH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HADH (hydroxyacyl-CoA dehydrogenase)This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineXenopus laevis (82%), Pig (95%), Chicken (80%), Rhesus Monkey (98%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HAD antibody HADH1 antibody HADHSC antibody HCDH antibody HHF4 antibody MGC8392 antibody MSCHAD antibody SCHAD antibody HADH antibody L-3-hydroxyacyl-Coenzyme A dehydrogenase short chain antibody hydroxyacyl-coenzyme A dehydrogenase mitochondri |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 μg of HADH antibody . Western blot analysis was performed using HADH antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
HADH antibody detects HADH protein at mitochondria on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HADH antibody dilution: 1:500.
Application
HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody dilution: 1:500.
Application
HADH antibody detects HADH protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE HADH antibody dilution: 1:1000
Application
HADH antibody detects HADH protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE HADH antibody dilution: 1:1000
Application
HADH antibody detects HADH protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HADH protein stained by HADH antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
HADH antibody detects HADH protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE HADH antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , GL261 , C8D30 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
