Application
Western blot analysis of extracts of various cell lines, using HADHA antibody.Application
Immunohistochemistry of paraffin-embedded human liver cancer using HADHA antibody at dilution of 1:100 (x40 lens).Application
Immunohistochemistry of paraffin-embedded human esophageal cancer using HADHA antibody at dilution of 1:100 (x40 lens).Application
Immunofluorescence analysis of U2OS cell using HADHA antibody. Blue: DAPI for nuclear staining.| Product Name | HADHA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HADHAThis gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human HADHA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ECHA TP-ALPHA HADH LCHAD LCEH MTPA hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein) alpha subunit GBP |
|---|---|
| Target | HADHA |
| Molecular Weight(MW) | 83 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using HADHA antibody.
Application
Immunohistochemistry of paraffin-embedded human liver cancer using HADHA antibody at dilution of 1:100 (x40 lens).
Application
Immunohistochemistry of paraffin-embedded human esophageal cancer using HADHA antibody at dilution of 1:100 (x40 lens).
Application
Immunofluorescence analysis of U2OS cell using HADHA antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
