Application
HAT1 antibody immunoprecipitates HAT1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HAT1 protein by 2 μg of HAT1 antibody 12% SDS-PAGE The immunoprecipitated HAT1 protein was detected by HAT1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HAT1 antibody [N1C3-2] detects HAT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HAT1 protein stained by HAT1 antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human colon, using HAT1 antibody at 1:250 dilution.| Product Name | HAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HAT1 (histone acetyltransferase 1)Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Full length human HAT1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (92%), Rat (95%), Chicken (83%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KAT1 antibody HAT1 antibody histone acetyltransferase type B catalytic subunit antibody histone acetyltransferase 1 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
HAT1 antibody immunoprecipitates HAT1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HAT1 protein by 2 μg of HAT1 antibody 12% SDS-PAGE The immunoprecipitated HAT1 protein was detected by HAT1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HAT1 antibody [N1C3-2] detects HAT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HAT1 protein stained by HAT1 antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human colon, using HAT1 antibody at 1:250 dilution.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
