Application
HAUS1(CCDC5 ) antibody [N1C3] detects HAUS1(CCDC5 ) protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HAUS1(CCDC5 ) protein stained by HAUS1(CCDC5 ) antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells.| Product Name | HAUS1(CCDC5 ) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HAUS1(CCDC5 ) (HAUS augmin-like complex, subunit 1)HAUS1 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM] |
| Immunogen | Full length human HAUS1(CCDC5 ) Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (84%), Rat (85%), Pig (88%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCDC5 antibody FLJ21094 antibody FLJ40084 antibody HEI-C antibody HEIC antibody HsT1461 antibody HAUS1 antibody coiled-coil domain containing 5 (spindle associated) antibody enhancer of invasion-cluster antibody coiled-coil domain-containing prote |
|---|---|
| Target | HAUS1(CCDC5 ) |
| Molecular Weight(MW) | 32 kDa (note) |
| Cellular Localization | Cytoplasm , Centrosome , Spindle |
Application
HAUS1(CCDC5 ) antibody [N1C3] detects HAUS1(CCDC5 ) protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HAUS1(CCDC5 ) protein stained by HAUS1(CCDC5 ) antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
