Application
HAUS6 antibody detects HAUS6 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HAUS6 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HAUS6 antibody detects HAUS6 protein on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. HAUS6 antibody dilution: 1:500.Application
HAUS6 ( IFA using at 1: 500; red) co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP; green) in U2OS cells. DAPI staining (blue) shows mitotic chromosomes. Arrows indicate spindle poles.| Product Name | HAUS6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HAUS6 (HAUS augmin-like complex, subunit 6)HAUS6 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HAUS6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Dgt6 antibody FAM29A antibody MGC102696 antibody MGC138798 antibody MGC138799 antibody HAUS6 antibody dim gamma-tubulin homolog antibody HAUS augmin-like complex subunit 6 antibody family with sequence similarity 29 member A antibody HAUS augmin- |
|---|---|
| Molecular Weight(MW) | 109 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton , Spindle , Centrosome |
Application
HAUS6 antibody detects HAUS6 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HAUS6 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HAUS6 antibody detects HAUS6 protein on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. HAUS6 antibody dilution: 1:500.
Application
HAUS6 ( IFA using at 1: 500; red) co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP; green) in U2OS cells. DAPI staining (blue) shows mitotic chromosomes. Arrows indicate spindle poles.| Positive Control | Jurkat , 293T , A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
