Application
Western blot analysis of HBP1 expression in SKBR3 (A); U937 (B) whole cell lysates.Application
Immunohistochemical analysis of HBP1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | HBP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HBP1 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HBP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HMG-box transcription factor 1 |
|---|---|
| Target | HBP1 |
| Tissue Specificity | Recognizes endogenous levels of HBP1 protein. |
Application
Western blot analysis of HBP1 expression in SKBR3 (A); U937 (B) whole cell lysates.
Application
Immunohistochemical analysis of HBP1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
