Rabbit Polyclonal antibody to HCAP-G (non-SMC condensin I complex, subunit G)Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Immunogen
Recombinant protein encompassing a sequence within the center region of human HCAP-G. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Predicted Reactivity
BovineBovine (81%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
NCAPG , non-SMC condensin I complex, subunit G , NY-MEL-3 , CHCG , CAPG
Target
HCAP-G
Molecular Weight(MW)
114 kDa (note)
Cellular Localization
Nucleus , Cytoplasm
Application
Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CAPG antibody diluted at1:2000.
Positive Control
293T , A431
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.