Application
Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 C: MOLT4 7.5% SDS PAGE diluted at 1:1000| Product Name | HCE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HCE (RNA guanylyltransferase and 5'-phosphatase)Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HCE. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (95%), Rat (96%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAP1A antibody, DKFZp686J2031 antibody, HCE antibody, HCE1 antibody, hCAP antibody, RNGTT antibody, mRNA-capping enzyme antibody, HCAP1 antibody, RNA guanylyltransferase and 5'-phosphatase antibody |
|---|---|
| Target | HCE |
| Molecular Weight(MW) | 69 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 C: MOLT4 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
