Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
A549 whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HCF1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HCF1 (host cell factor C1 (VP16-accessory protein))This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HCF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevisMouse (99%), Xenopus laevis (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CFF antibody, HCF-1 antibody, HCF1 antibody, HFC1 antibody, MGC70925 antibody, VCAF antibody, HCFC1 antibody, C1 factor antibody, VP16 accessory protein antibody, HCF antibody, host cell factor 1 antibody, host cell factor C1 (VP16-accessory protein) antib |
|---|---|
| Molecular Weight(MW) | 209 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
A549 whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , A549 , A549 nuclear extract , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
