Application
Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr522) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | HCK (phospho Tyr521) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HCK (phospho Tyr521)The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 522(S-Q-Y(p)-Q-Q) derived from Human HCK . |
| Modification | p-Tyr521 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HCK p61Hck HCK proto-oncogene Src family tyrosine kinase p59Hck JTK9 |
|---|---|
| Target | HCK (phospho Tyr521) |
| Tissue Specificity | The antibody detects endogenous levels of HCK only when phosphorylated at tyrosine 522. |
Application
Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr522) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
