Application
Immunohistochemical analysis of paraffin - embedded human colon cancer (top) and pancreas cancer (bottom) showing cytoplasma localization using HCK antibody with DAB staining.Application
Western blot analysis using HCK monoclonal antibody against truncated HCK - His recombinant protein and full - length HCK / GFP transfected CHO - K1 cell lysate.| Product Name | HCK Antibody [3D12E10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Hemopoietic cell kinase.The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.1) J Clin Gastroenterol. 2007 Aug;41(7):667-70. |
| Immunogen | Ni-NTA purified truncated recombinant HCK-His fusion protein is expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HCK |
|---|---|
| Alternative Names | Hematopoietic cell kinaseJTK9 p59Hck p61Hck |
Application
Immunohistochemical analysis of paraffin - embedded human colon cancer (top) and pancreas cancer (bottom) showing cytoplasma localization using HCK antibody with DAB staining.
Application
Western blot analysis using HCK monoclonal antibody against truncated HCK - His recombinant protein and full - length HCK / GFP transfected CHO - K1 cell lysate.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | HCK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
