Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE HCLS1 antibody diluted at 1:1000Application
HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 μg of HCLS1 antibody 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | HCLS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HCLS1 (hematopoietic cell-specific Lyn substrate 1)Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HCLS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, BovineMouse (95%), Rat (95%), Chicken (82%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTTNL antibody HS1 antibody HCLS1 antibody hematopoietic lineage cell-specific protein antibody lckBP1 antibody p75 antibody hematopoietic cell-specific Lyn substrate 1 antibody |
|---|---|
| Target | HCLS1 |
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Membrane; Peripheral membrane protein (Probable) , Cytoplasm (Probable) , Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE HCLS1 antibody diluted at 1:1000
Application
HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 μg of HCLS1 antibody 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
