Application
Western blot analysis of Histone Deacetylase 1 (pS421) expression in MCF7 EGF-treated (A); Jurkat (B); NIH3T3 (C); mouse spleen (D); H9C2 EGF-treated (E) whole cell lysates.| Product Name | HDAC1 (phospho Ser421) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC1 (phospho Ser421)Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone Deacetylase 1. The exact sequence is proprietary. |
| Modification | p-Ser421 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HDAC1 , GON-10 , RPD3 , HD1 , histone deacetylase 1 , RPD3L1 |
|---|---|
| Target | HDAC1 (phospho Ser421) |
| Tissue Specificity | Recognizes endogenous levels of Histone Deacetylase 1 (pS421) protein. |
Application
Western blot analysis of Histone Deacetylase 1 (pS421) expression in MCF7 EGF-treated (A); Jurkat (B); NIH3T3 (C); mouse spleen (D); H9C2 EGF-treated (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
