Application
IHC-P analysis of human breast carcinoma tissue using HDAC1 (phospho Ser421/Ser423) antibody.Application
WB analysis of extracts from HELA cells using HDAC1 (phospho Ser421/Ser423) antibody. Additional analysis with blocking peptide pre-incubated HDAC1 (phospho Ser421/Ser423) antibody was also perform (right).| Product Name | HDAC1 (phospho Ser421/Ser423) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HDAC1 (phospho S421/S423)Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq] |
| Modification | p-Ser421/Ser423 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1 |
|---|---|
| Target | HDAC1 (phospho S421/S423) |
Application
IHC-P analysis of human breast carcinoma tissue using HDAC1 (phospho Ser421/Ser423) antibody.
Application
WB analysis of extracts from HELA cells using HDAC1 (phospho Ser421/Ser423) antibody. Additional analysis with blocking peptide pre-incubated HDAC1 (phospho Ser421/Ser423) antibody was also perform (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
