Application
Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with to HDAC1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2| Product Name | HDAC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HDAC1 - ChIP GradeReversible acetylation of highly conserved lysine residues in N-terminal tail domains of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone acetylation is a dynamic process determined by the net activities of histone acetyltransferases (HATs) and the competing enzymes histone deacetylases (HDACs) (ref 3). Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations (ref 6,7). Recruitment of the multiprotein complexes to promoter sites occurs by many sequence specific DNA-binding proteins such as unliganded nuclear hormone receptors, DP1-E2F, YY1 and Rb family of transcription factors, transcriptional repressors and tumor suppressors (e.g. BRCA1). Aberrant recruitment of HDACs by certain oncoproteins may occur in certain neoplastic diseases.(ref 11) |
| Immunogen | Synthetic peptide: C-KEEKPEAKGVKEEVKLA conjugated to KLH, corresponding to amino acids 466-482 of Human HDAC 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotIHC-PIPWBChIP |
| ChIP: Use 5μl for 10^6 cells. Dot: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IP: Use at a dilution of 1/2500. WB: Use at a dilution of 1/2000 - 1/20000. Predicted molecular weight: 55 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | HDAC1, GON-10, DKFZp686H12203, HD1, RPD3, 601241, GON10, RPD3L1, 3065, Q13547, GON 10 |
|---|---|
| Target | HDAC1 |
| Tissue Specificity | Anti-Histone Deacetylase 1 specifically recognizes histone deacetylase 1 by immunoblotting and immunoprecipitation (65 kD). |
| Cellular Localization | Nuclear |
Application
Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with to HDAC1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2| Application Notes | ChIP: Use 5μl for 10^6 cells. Dot: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IP: Use at a dilution of 1/2500. WB: Use at a dilution of 1/2000 - 1/20000. Predicted molecular weight: 55 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS pH7.4, 15mM Na azide |
