Application
Western blot analysis of HDAC-1 expression with lysates from mouse small intestine (Lane 1), Jurkat cells (Lane 2), and rat kidney (Lane 3).| Product Name | HDAC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HDAC1HDAC1 is an active component of transcriptional corepressor complexes which can be recruited to specific promoter regions via transcriptional factors. HDAC1 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, which leads to the inhibition of gene transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| Western blotting (0.5-4 µg/ml), Immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 62 kDa histone deacetylase 1. It does not cross-react with other HDAC proteins including HDAC2, 3, 4, 5, 6, 7, and 8. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HDAC1, Like-1, Histone Deacetylase 1, Like1, Reduced Potassium Dependency3, HD1, RPD3, Reduced Potassium Dependency 3, RPD3L1, HD-1, Histone Deacetylase1, HDAC-1, HD 1, Like 1, HDAC 1 |
|---|---|
| Target | HDAC1 |
| Tissue Specificity | This antibody is specific for HDAC1. |
Application
Western blot analysis of HDAC-1 expression with lysates from mouse small intestine (Lane 1), Jurkat cells (Lane 2), and rat kidney (Lane 3).| Application Notes | Western blotting (0.5-4 µg/ml), Immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 62 kDa histone deacetylase 1. It does not cross-react with other HDAC proteins including HDAC2, 3, 4, 5, 6, 7, and 8. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
