Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody 10% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC3 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term diluted at 1:400. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HDAC3 antibody at 1:500 dilution.| Product Name | HDAC3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC3 (histone deacetylase 3)Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, Rhesus Monkey, BovineRat (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HD3 antibody RPD3 antibody RPD3-2 antibody HDAC3 antibody SMAP45 antibody histone deacetylase 3 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody 10% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC3 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term diluted at 1:400. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HDAC3 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS , *FaDu |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
