Application
Western blot analysis of extracts of various cell lines, using HDAC4 antibody.Application
Immunofluorescence analysis of A549 cell using HDAC4 antibody. Blue: DAPI for nuclear staining.| Product Name | HDAC4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC4Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide of human HDAC4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:50 - 1:200, ChIP 1:20 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HA6116 , HDAC-4 , HDAC-A , AHO3 , BDMR , histone deacetylase 4 , HDACA , HD4 |
|---|---|
| Target | HDAC4 |
| Molecular Weight(MW) | 119 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using HDAC4 antibody.
Application
Immunofluorescence analysis of A549 cell using HDAC4 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:50 - 1:200, ChIP 1:20 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
