Application
Western blot analysis of HDAC-4 in mouse brain tissue lysate. A 140 kDa band can be detected.| Product Name | HDAC4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HDAC4 - ChIP GradeHDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C-terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART. |
| Immunogen | Unfortunately, this information is considered to be commercially sensitive |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBChIP |
| ChIP: Use 6μl for 10^6 cells. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 119 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 605314, HD4, HDAC-A, HDAC4, KIAA0288, 9759, P56524, HA6116, HDACA, AHO3, BDMR, HDAC A |
|---|---|
| Target | HDAC4 |
| Tissue Specificity | The antibody detects ~140 kDa histone deacetylase 4. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 5, 6, 7, and 8. |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Western blot analysis of HDAC-4 in mouse brain tissue lysate. A 140 kDa band can be detected.| Application Notes | ChIP: Use 6μl for 10^6 cells. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 119 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C |
| Storage Buffer | PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide |
