Application
HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HDAC4 protein by 4 μg HDAC4 antibody [N3C1], Internal 5 % SDS-PAGE The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC4 antibody at 1:250 dilution.| Product Name | HDAC4 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC4 (histone deacetylase 4)Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HDAC4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, ChickenMouse (90%), Chicken (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AHO3 antibody, BDMR antibody, HA6116 antibody, HD4 antibody, HDAC-A antibody, HDACA antibody, KIAA0288 antibody, HDAC4 antibody, histone deacetylase 4 antibody, histone deacetylase A antibody |
|---|---|
| Molecular Weight(MW) | 119 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HDAC4 protein by 4 μg HDAC4 antibody [N3C1], Internal 5 % SDS-PAGE The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC4 antibody at 1:250 dilution.| Positive Control | 293T , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
