Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5 (Phospho-Ser498) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5 (Phospho-Ser498) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5(Phospho-Ser498) Antibody| Product Name | HDAC5 (phospho Ser498) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC5 (Phospho Ser498)Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Peptide sequence around phosphorylation site of serine 498 (T-Q-S(p)-S-P) derived from Human HDAC5/7. |
| Modification | p-Ser498 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ90614, HD5, NY-CO-9, HDAC5, histone deacetylase 5, antigen NY-CO-9 |
|---|---|
| Target | HDAC5 (Phospho Ser498) |
| Molecular Weight(MW) | 124 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5 (Phospho-Ser498) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5 (Phospho-Ser498) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5(Phospho-Ser498) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
