Application
Western blot analysis of HDAC5 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | HDAC5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC5Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human HDAC5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HD5, histone deacetylase 5, NY-CO-9 |
|---|---|
| Target | HDAC5 |
| Tissue Specificity | HDAC5 |
Application
Western blot analysis of HDAC5 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
