Application
Western blot analysis of HDAC5 expression in Jurkat cell lysate| Product Name | HDAC5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HDAC5HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| IHC-P: Use at a concentration of 20 μg/ml. IP: Use at a concentration of 20 μg/ml. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 121.9 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 605315, HD5, NYCO9, 10014, NY-CO-9, HDAC5, Q9UQL6, FLJ90614, NY CO 9 |
|---|---|
| Target | HDAC5 |
| Tissue Specificity | The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8. |
| Cellular Localization | Nuclear and cytoplasmic. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its p |
Application
Western blot analysis of HDAC5 expression in Jurkat cell lysate| Positive Control | HeLa or COS cell lysate can be used as the positive controls |
|---|---|
| Application Notes | IHC-P: Use at a concentration of 20 μg/ml. IP: Use at a concentration of 20 μg/ml. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 121.9 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C |
| Storage Buffer | PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide |
