Application
Whole extract of HEK-293T cells overexpressing human HDAC6 was separated on SDS-PAGE and probed with Rabbit Anti-HDAC6 . The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 1:1,000 antibody 2. 1:1,000 antibody + HDAC6 immunizing peptide| Product Name | HDAC6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HDAC6Regulation of gene expression is mediated by several mechanisms. Among them are DNA methylation, ATP dependent chromatin remodeling, and post-translational modifications of histones, such as the dynamic acetylation and deacetylation of epsilon-amino groups of lysine residues present in the tail of core histones. The enzymes responsible for this reversible acetylation/deacetylation process are histone acetyltransferases(HATs) and histone deacetylases (HDACs), respectively. HATs act as transcriptional coactivators and HDACs are part of transcriptional corepressor complexes. Mammalian HDACs can be divided into three classesaccording to sequence homology. Class I consists of the yeast Rpd3-like proteins HDAC1, HDAC2, HDAC3 and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Class III consists of the yeast Sir2-like proteins. Whereas class I HDACs are ubiquitously expressed, most class II HDACs are tissue-specific. Class II HDACs are larger than those of class I and their catalytic domain is located in the carboxy terminal half of the protein. HDAC6 is a unique deacetylase among class II members in that it contains a duplicate of the catalytic domain in its NH2 terminus as well. The deacetylase activity of class II HDACs is regulated by subcellular localization. Endogenous HDAC6 is predominantly cytoplasmic where it associates with microtubules and functions as an alpha-tubulin deacetylase. HDAC6 contains a conserved zinc finger motif that is probably involved in the regulation of ubiquitination. |
| Immunogen | Synthetic peptide: KNIAHQNKFGEDMPH conjugated to KLH, corresponding to amino acids 1199-1213 of Human HDAC6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 134 kDa. Additional non-specific bands of approx. 50 kDa may be detected in various extract preparations. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | HDAC6, 10013, 300272, Q9UBN7, JM21, HD6 |
|---|---|
| Target | HDAC6 |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Whole extract of HEK-293T cells overexpressing human HDAC6 was separated on SDS-PAGE and probed with Rabbit Anti-HDAC6 . The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 1:1,000 antibody 2. 1:1,000 antibody + HDAC6 immunizing peptide| Positive Control | 293T cells expressing recombinant mouse HDAC6 |
|---|---|
| Application Notes | IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 134 kDa. Additional non-specific bands of approx. 50 kDa may be detected in various extract preparations. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline containing 15 mM sodium azide. |
