Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
HDAC6 antibody detects HDAC6 protein by western blot analysis. Non-transfected (-) and HDAC6-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC6 antibody diluted by 1:5000.| Product Name | HDAC6 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC6 (histone deacetylase 6)Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HDAC6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16239 antibody, HD6 antibody, HDAC6 antibody, histone deacetylase 6 antibody |
|---|---|
| Molecular Weight(MW) | 131 kDa (note) (8) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
HDAC6 antibody detects HDAC6 protein by western blot analysis. Non-transfected (-) and HDAC6-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC6 antibody diluted by 1:5000.| Positive Control | 3xFlag-human HDAC6-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
