Application
Sample lysates were prepared from HEK 293T cells expressing DDDDK-tagged human HDAC7. The precipitated hHDAC7 was detected using anti-DDDDK Tag antibody and visualized by HRP-conjugated goat anti-rabbit secondary antibody with enhanced chemiluminescent reagent. Lane 1: 2 µg Anti-HDAC7 antibody [KG-17] Lane 2: 2 µg control rabbit IgG Each sample contains 180µg whole cell lysate.Application
Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17) . The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HeLa nuclear 2. NIH-3T3| Product Name | HDAC7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HDAC7HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription. |
| Immunogen | Synthetic peptide: RSEPLPPSATAPPPPGP conjugated to KLH, corresponding to amino acids 357-373 of Human HDAC7. The corresponding sequence is identical in mouse. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| IF: Use at a dilution of 1/50. This dilution is recommended using rat normal rat kidney (NRK) cells. IP: Use at an assay dependent dilution. 1.0-1.5 μg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 μg) of 293T cells expressing recombinant human HDAC7. WB: Use at a dilution of 1/1000. This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Predicted molecular weight: 105 kDa. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZP586J0917, Q8WUI4, 51564, HDAC7A, 606542, HDAC7 |
|---|---|
| Target | HDAC7 |
| Cellular Localization | Nuclear and cytoplasmic |
Application
Sample lysates were prepared from HEK 293T cells expressing DDDDK-tagged human HDAC7. The precipitated hHDAC7 was detected using anti-DDDDK Tag antibody and visualized by HRP-conjugated goat anti-rabbit secondary antibody with enhanced chemiluminescent reagent. Lane 1: 2 µg Anti-HDAC7 antibody [KG-17] Lane 2: 2 µg control rabbit IgG Each sample contains 180µg whole cell lysate.
Application
Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17) . The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HeLa nuclear 2. NIH-3T3| Positive Control | Mouse NIH-3T3 cells (Western blotting)Normal rat kidney (NRK) cells (indirect immunofluorescence) |
|---|---|
| Application Notes | IF: Use at a dilution of 1/50. This dilution is recommended using rat normal rat kidney (NRK) cells. IP: Use at an assay dependent dilution. 1.0-1.5 μg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 μg) of 293T cells expressing recombinant human HDAC7. WB: Use at a dilution of 1/1000. This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Predicted molecular weight: 105 kDa. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC. Avoid freeze-thaw cycles. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
