Application
Immunofluorescence analysis of paraformaldehyde-fixed HCT116, using HDAC7 antibody at 1:500 dilution.Application
Sample (20 ug of whole cell lysate) A: 293T transfected HDAC7-FLAG diluted at 1:2000| Product Name | HDAC7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC7 (histone deacetylase 7)Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HDAC7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Chicken, BovineMouse (97%), Chicken (90%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586J0917 antibody, FLJ99588 antibody, HD7A antibody, HDAC7A antibody, HDAC7 antibody, histone deacetylase 7 antibody, histone deacetylase 7A antibody, HD7 antibody |
|---|---|
| Molecular Weight(MW) | 103 kDa (note) (4) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HCT116, using HDAC7 antibody at 1:500 dilution.
Application
Sample (20 ug of whole cell lysate) A: 293T transfected HDAC7-FLAG diluted at 1:2000| Positive Control | HDAC7-FLAG tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
