Application
Immunofluorescence staining of methanol-fixed Hela cells using HDAC8 (Phospho-Ser39) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8 (Phospho-Ser39) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | HDAC8 (phospho Ser39) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC8 (Phospho Ser39)Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC8 gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Peptide sequence around phosphorylation site of serine 39 (R-A-S(p)-M-V) derived from Human HDAC8. |
| Modification | p-Ser39 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HD8, HDACL1, RPD3, HDAC8, histone deacetylase 8, histone deacetylase-like 1 |
|---|---|
| Target | HDAC8 (Phospho Ser39) |
| Molecular Weight(MW) | 42 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using HDAC8 (Phospho-Ser39) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8 (Phospho-Ser39) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
