Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC8 antibody diluted at 1:1000.Application
HDAC8 antibody detects HDAC8 protein at cytoplasm and nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. HDAC8 antibody diluted at 1:500.Application
HDAC8 antibody detects HDAC8 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HDAC8 antibody diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:5000Application
HDAC8 antibody detects HDAC8 protein by Western blot analysis. A. 50 µg rat heart lysate/extract 10 % SDS-PAGE HDAC8 antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000Application
Immunoprecipitation of HDAC8 protein from 293T whole cell extracts using 5 μg of HDAC8 antibody . Western blot analysis was performed using HDAC8 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | HDAC8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDAC8 (histone deacetylase 8)Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HDAC8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, BovineXenopus laevis (88%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HD8 antibody HDACL1 antibody RPD3 antibody HDAC8 antibody histone deacetylase 8 antibody histone deacetylase-like 1 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC8 antibody diluted at 1:1000.
Application
HDAC8 antibody detects HDAC8 protein at cytoplasm and nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. HDAC8 antibody diluted at 1:500.
Application
HDAC8 antibody detects HDAC8 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HDAC8 antibody diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:5000
Application
HDAC8 antibody detects HDAC8 protein by Western blot analysis. A. 50 µg rat heart lysate/extract 10 % SDS-PAGE HDAC8 antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000
Application
Immunoprecipitation of HDAC8 protein from 293T whole cell extracts using 5 μg of HDAC8 antibody . Western blot analysis was performed using HDAC8 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , mouse brain , rat heart , *RAW 264.7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
