Rabbit polyclonal antibody to HDAC9HDAC family members have been divided into two classes. Class I of the HDACs comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDACs comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
Western blotting (0.5-4 µg/ml), Immunoprecipitation and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes a ~110-140 kDa of HDAC-9. A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.:
Western blot analysis of HDAC-9 in mouse small intestine tissue lysate.
Application Notes
Western blotting (0.5-4 µg/ml), Immunoprecipitation and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes a ~110-140 kDa of HDAC-9. A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.:
Additional Information
Form
Liquid
Storage Instructions
The antibody solution should be gently mixed before use. Stored at -20 °C for future use.