Application
HDGF antibody detects HDGF protein at cytoplasm and nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HDGF antibody diluted at 1:500.Application
HDGF antibody detects HDGF protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. HDGF antibody diluted at 1:500.Application
HDGF antibody detects HDGF protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDGF protein stained by HDGF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HDGF antibody diluted at 1:5000.| Product Name | HDGF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDGF (hepatoma-derived growth factor)This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq] |
| Immunogen | Full length human HDGF recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686J1764 antibody FLJ96580 antibody HDGF antibody HDGF antibody HMG-1L2 antibody HMG1L2 antibody hepatoma-derived growth factor antibody high mobility group protein 1-like 2 antibody |
|---|---|
| Target | HDGF |
| Molecular Weight(MW) | 27 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
HDGF antibody detects HDGF protein at cytoplasm and nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HDGF antibody diluted at 1:500.
Application
HDGF antibody detects HDGF protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. HDGF antibody diluted at 1:500.
Application
HDGF antibody detects HDGF protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDGF protein stained by HDGF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HDGF antibody diluted at 1:5000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
