Application
WB analysis of extracts from various cell lines using HDGF antibody at a dilution of 1:1000.Application
IHC-P analysis of human breast cancer using HDGF antibody at a dilution of 1:200.Application
IHC-P analysis of human stomach tissue using HDGF antibody at a dilution of 1:200.Application
Immunofluorescence analysis of U20S cell using HDGF antibody (red) at a dilution of 1:50 and DAPI (blue).Application
ICC/IF analysis of A549 cells using HDGF antibody at a dilution of 1:50.| Product Name | HDGF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HDGFThis gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human HDGF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPRIPWB |
| Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:2000.
For IHC-P: Use at a dilution of 1:50-1:200.
For ICC/IF: Use at a dilution of 1:50-1:200.
For IP: Use at a dilution of 1:20-1:50.
For RIP: Use at a dilution of 1:20-1:50.
Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hepatoma-derived growth factor HMG1L2 |
|---|---|
| Target | HDGF |
| Molecular Weight(MW) | 26 kDa (note) |
Application
WB analysis of extracts from various cell lines using HDGF antibody at a dilution of 1:1000.
Application
IHC-P analysis of human breast cancer using HDGF antibody at a dilution of 1:200.
Application
IHC-P analysis of human stomach tissue using HDGF antibody at a dilution of 1:200.
Application
Immunofluorescence analysis of U20S cell using HDGF antibody (red) at a dilution of 1:50 and DAPI (blue).
Application
ICC/IF analysis of A549 cells using HDGF antibody at a dilution of 1:50.| Application Notes | Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:2000.
For IHC-P: Use at a dilution of 1:50-1:200.
For ICC/IF: Use at a dilution of 1:50-1:200.
For IP: Use at a dilution of 1:20-1:50.
For RIP: Use at a dilution of 1:20-1:50.
Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
