Application
Immunofluorescence analysis of methanol-fixed HeLa, using HEBP1 antibody at 1:400 dilution.Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using HEBP1 antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000Application
HEBP1 antibody [N1C3] detects HEBP1 protein at cytoplasm in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. HEBP1 antibody [N1C3] diluted at 1:500.Application
HEBP1 antibody [N1C3] detects HEBP1 protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HEBP1 antibody [N1C3] diluted at 1:500.| Product Name | HEBP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HEBP1 (heme binding protein 1)The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HEBP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (84%), Rhesus Monkey (98%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HBP antibody HEBP antibody HEBP1 antibody p22HBP antibody heme-binding protein 1 antibody heme binding protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using HEBP1 antibody at 1:400 dilution.
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using HEBP1 antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000
Application
HEBP1 antibody [N1C3] detects HEBP1 protein at cytoplasm in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. HEBP1 antibody [N1C3] diluted at 1:500.
Application
HEBP1 antibody [N1C3] detects HEBP1 protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HEBP1 antibody [N1C3] diluted at 1:500.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
