| Product Name | HEF 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2G9] to HEF 1The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Immunogen | GST-HEF-1(82-398aa.) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2G9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| For WB: Use at 1:2000-1:6000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | NEDD9 CASS2 CAS-L HEF1 CASL CAS2 |
|---|---|
| Target | HEF 1 |
| Tissue Specificity | Human, mouse, rat HEF-1. Does not recognize p130Cas. Have not tested on Sin1. This mAb mostly detects HEF1 localized at the focal adhesion sites |
| Cellular Localization | Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. |
| Light Chain Type | kappa |
| Application Notes | For WB: Use at 1:2000-1:6000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS + 0.1% sodium azide |
