| Product Name | HEF-1/Cas-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [14A11] to HEF-1/Cas-LThe protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Immunogen | GST-HEF-1 (82-398aa.) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 14A11 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIP |
| at 1μg of antibody per 200μg of whole cell lysate for IP, & 1:100 for IF: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | NEDD9 CASS2 CAS-L HEF1 CASL CAS2 |
|---|---|
| Target | HEF-1/Cas-L |
| Tissue Specificity | Specific for human HEF1 localized in mitotic cells in the spindle and the centrosome. It does not recognize p130Cas. Have not tested it on Sin1 |
| Cellular Localization | Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. |
| Light Chain Type | kappa |
| Application Notes | at 1μg of antibody per 200μg of whole cell lysate for IP, & 1:100 for IF: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS + 0.1% sodium azide |
