Application
Formalin fixed paraffin embedded human stomach infected with H. pylori and stained with H. pylori antibody (cat. # ).Application
IHC Image using - Detection of Helicobactor Pylori by IHC in Human Stomach| Product Name | Helicobactor Pylori antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyclonal Antibody to Helicobactor PyloriThis antibody reacts with the whole H. pylori. The antibody stains the bacteria present on the surface of the epithelium or in the cytoplasm of the epithelial cells. H. pylori is known to cause peptic ulcer and chronic gastritis in human. |
| Immunogen | Total lysate of H. pylori were injected to the rabbit. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Helicobacter pylori |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Helicobactor Pylori |
|---|---|
| Cellular Localization | Cytoplasmic |
Application
Formalin fixed paraffin embedded human stomach infected with H. pylori and stained with H. pylori antibody (cat. # ).
Application
IHC Image using - Detection of Helicobactor Pylori by IHC in Human Stomach| Positive Control | Infected stomach |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
