Application
HELIOS antibody detects HELIOS protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HELIOS antibody dilution: 1:500.Application
IKZF2 antibody detects IKZF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IKZF2 protein stained by IKZF2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody diluted by 1:1000.| Product Name | HELIOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HELIOS (IKAROS family zinc finger 2 (Helios))This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HELIOS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, Rhesus MonkeyRat (81%), Chicken (92%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ANF1A2 ZNFN1A2 ZNF1A2 IKAROS family zinc finger 2 (Helios) IKZF2 |
|---|---|
| Target | HELIOS |
| Molecular Weight(MW) | 60 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
HELIOS antibody detects HELIOS protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HELIOS antibody dilution: 1:500.
Application
IKZF2 antibody detects IKZF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IKZF2 protein stained by IKZF2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody diluted by 1:1000.| Positive Control | Jurkat , Jurkat NE |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
