Application
Flow cytometric analysis of MCF-7 cells using ERBB2 mouse mAb ( green) and negative control (purple).Application
Immunohistochemical analysis of paraffin-embedded galactophore tumour using ERBB2 mouse mAb with DAB stainingApplication
Western blot analysis using ERBB2 mouse mAb against SKBR3 (1) and MCF-7 (2) cell lysate| Product Name | Her2 / ErbB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6C2] to ErbB2This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 750 and 987 of human ERBB2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6C2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TKR1 NEU NGL CD340 HER-2 HER2 ERBB2 HER-2/NEU MLN 19 |
|---|---|
| Target | ERBB2 |
| Molecular Weight(MW) | 180 kDa (note) |
Application
Flow cytometric analysis of MCF-7 cells using ERBB2 mouse mAb ( green) and negative control (purple).
Application
Immunohistochemical analysis of paraffin-embedded galactophore tumour using ERBB2 mouse mAb with DAB staining
Application
Western blot analysis using ERBB2 mouse mAb against SKBR3 (1) and MCF-7 (2) cell lysate| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
