Application
Confocal immunofluorescence analysis of HEK293 cells trasfected with extracellular ERBB3 (aa22-369)-hIgGFc using anti-ERBB3 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.Application
Western blot analysis using anti-ERBB3 monoclonal antibody against human ERBB3 (aa22-369)-hIgGFc trasfected HEK293 cell lysate (1).| Product Name | Her3 / ErbB3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2F9] to ERBB3This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughl |
| Immunogen | The immunogen corresponding to a region within amino acids 22 and 369 of human ERBB3 fused with hIgGFc tag expressed in HEK293 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F9 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P85-SERBB3 LCCS2 C-ERBB3 ERBB-3 C-ERBB-3 P180-ERBB3 P45-SERBB3 MDA-BF-1 HER3 ERBB3-S |
|---|---|
| Target | ERBB3 |
| Molecular Weight(MW) | 148 kDa (note) |
Application
Confocal immunofluorescence analysis of HEK293 cells trasfected with extracellular ERBB3 (aa22-369)-hIgGFc using anti-ERBB3 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Western blot analysis using anti-ERBB3 monoclonal antibody against human ERBB3 (aa22-369)-hIgGFc trasfected HEK293 cell lysate (1).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
