Application
Flow cytometry analysis of ErbB3 in HeLa cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ErbB3 antibody [RTJ2] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.| Product Name | Her3 / ErbB3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [RTJ2] to ErbB3This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughl |
| Immunogen | Synthetic Peptide: E(1004) L E P E L D L D L D L E(1016) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | RTJ2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PIPWB |
| IHC-P: 5 µg/ml: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) c-erbB-3 LCCS2 c-erbB3 ERBB3 p85-sErbB3 p45-sErbB3 ErbB-3 p180-ErbB3 MDA-BF-1 H |
|---|---|
| Target | ErbB3 |
Application
Flow cytometry analysis of ErbB3 in HeLa cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ErbB3 antibody [RTJ2] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.| Application Notes | IHC-P: 5 µg/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide and 1 mg/ml BSA |
