Application
Western blot analysis of extracts from HUVEC cells treated with EGF using HER4 (Phospho-Tyr1284) Antibody. The lane on the left is treated with the antigen-specific peptide.| Product Name | Her4 / ErbB4 (phospho Tyr1284) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERBB4 (phospho Tyr1284)This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 1284 (P-E-Y(p)-L-S) derived from Human HER4 |
| Modification | p-Tyr1284 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HER4 erb-b2 receptor tyrosine kinase 4 ALS19 p180erbB4 ERBB4 |
|---|---|
| Target | ERBB4 (phospho Tyr1284) |
| Tissue Specificity | The antibody detects endogenous level of p38 HER4 only when phosphorylated at Tyrosine 1284. |
Application
Western blot analysis of extracts from HUVEC cells treated with EGF using HER4 (Phospho-Tyr1284) Antibody. The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
