Application
ERBB4 antibody [C1C3] immunoprecipitates ERBB4 protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of ERBB4 protein by 4 μg ERBB4 antibody [C1C3] 5 % SDS-PAGE The immunoprecipitated ERBB4 protein was detected by ERBB4 antibody [C1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4(v-erb-A) antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Her4 / ErbB4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERBB4 (v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian))This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ERBB4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, ChickenRat (97%), Xenopus laevis (86%), Chicken (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HER4 antibody MGC138404 antibody p180erbB4 antibody ERBB4 antibody avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4 antibody tyrosine kinase-type cell surface receptor HER4 antibody proto-oncogene-like protein c-ErbB-4 antibody rece |
|---|---|
| Molecular Weight(MW) | 147 kDa (note) (2) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
ERBB4 antibody [C1C3] immunoprecipitates ERBB4 protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of ERBB4 protein by 4 μg ERBB4 antibody [C1C3] 5 % SDS-PAGE The immunoprecipitated ERBB4 protein was detected by ERBB4 antibody [C1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4(v-erb-A) antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | A431 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
