| Product Name | Her4 / ErbB4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [H4.77.16] to ErbB 4C-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation. |
| Immunogen | Extracellular fragment of recombinant human c-erbB-4 / HER 4 oncoprotein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | H4.77.16 |
| Host Species | Mouse |
| Tested Applications | FACSIP |
| Flow Cytometry: assay dependent. Functional studies (see below): assay dependent. Immunoprecipitation (with protein G): use at a concentration of 2μg/mg of protein lysate. Optimal dilutions / concentrations should be determined by the end user. This antibody is not suitable for western blot (Tang C, et. al). This antibody does not interfere with receptor-heregulin binding and is particularly wellsuited for immunoprecipitation of unoccupied as wellas the occupied c-erbB-4 receptor (Chen X, et. al.). It reacts withintact cells, stimulates tyrosine kinase activity, andefficiently stimulates internalization of c-erbB-4 (Chen X, et. al.).: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ErbB4 HER4 2066 600543 Q15303 MGC138404 |
|---|---|
| Target | ErbB4 |
| Cellular Localization | Type I membrane protein |
| Positive Control | T47D or MDA-MB-453 cells |
|---|---|
| Application Notes | Flow Cytometry: assay dependent. Functional studies (see below): assay dependent. Immunoprecipitation (with protein G): use at a concentration of 2μg/mg of protein lysate. Optimal dilutions / concentrations should be determined by the end user. This antibody is not suitable for western blot (Tang C, et. al). This antibody does not interfere with receptor-heregulin binding and is particularly wellsuited for immunoprecipitation of unoccupied as wellas the occupied c-erbB-4 receptor (Chen X, et. al.). It reacts withintact cells, stimulates tyrosine kinase activity, andefficiently stimulates internalization of c-erbB-4 (Chen X, et. al.).: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 1X PBS buffer with 0.1% sodium azide |
