Application
Immunohistochemical analysis of paraffin-embedded U251 xenograft, using HERPUD1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:5000Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000| Product Name | HERPUD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HERPUD1 (homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1)The accumulation of unfolded proteins in the endoplasmic reticulum (ER) triggers the ER stress response. This response includes the inhibition of translation to prevent further accumulation of unfolded proteins, the increased expression of proteins involved in polypeptide folding, known as the unfolded protein response (UPR), and the destruction of misfolded proteins by the ER-associated protein degradation (ERAD) system. This gene may play a role in both UPR and ERAD. Its expression is induced by UPR and it has an ER stress response element in its promoter region while the encoded protein has an N-terminal ubiquitin-like domain which may interact with the ERAD system. This protein has been shown to interact with presenilin proteins and to increase the level of amyloid-beta protein following its overexpression. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. The full-length nature of all transcript variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HERPUD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (83%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HERP antibody KIAA0025 antibody Mif1 antibody SUP antibody HERPUD1 antibody homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein antibody homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded U251 xenograft, using HERPUD1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:5000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
